Isolation and Characterization of Biohydrogen-Producing Bacteria for Biohydrogen Fermentation Using Oil Palm Biomass-Based Carbon Source

The effectiveness of biohydrogen conversion from biomass sources is governed by the selection ideal biohydrogen-producing bacteria to achieve high and consistent production performance. aim this research was isolate identify a producer in local soil samples, as well evaluate its fermentability oil palm empty fruit bunches (OPEFB). To end, preliminary identification performed using morphological, phenotype, biological, 16s rRNA analyses. further evaluated serum bottle then 1.5 L anaerobic column bioreactor (ACBR) investigate potential for two OPEFB-based carbon sources: hydrolysate ammonia fiber expansion (AFEX)-pretreated OPEFB molasses dilute acetic acid (DAA)-pretreated OPEFB. isolated strain, Enterobacter sp. KBH 6958, found be capable producing various via pyruvate:ferredoxin oxidoreductase (PFOR) pathway. cumulative AFEX 45% higher than that observed DAA fermentation biohydrogen. yield after fermenting with 6958 1.55 mol H2/mol sugar, maximum productivity 98.1 mL H2/h (4.01 mmol H2/L/h), whereas butyrate (10.6 mM), acetate (11.8 ethanol (4.56 mM) were major soluble metabolites. This study successfully demonstrated biotechnological into locally which not only solves environmental issues associated industry but may also offer solution world’s energy insecurity.